Tumours with TLSs tend to have distinct features, including increased infiltration by CD20+ B cells and both CD8+ and CD4+ T cells (44, 47), decreased infiltration of CD163+ M2 macrophages (48), decreased expression of the Treg markers FOXP3 and CD25 by CD4+ T cells (48, 49), and higher levels of Bcl-2 and lower levels of TIM3 expressed by T cells (40). This evidence concerns the gene CD4 and neoplasm.