An integrated approach, named STARTRAC has been carried out to track the dynamic relationship among T cells in CRC and identified 8 CD8+ and 12 CD4+T cell clusters, including naïve, central memory, effector memory and recently activated effector memory or effector T cells, mucosal-associated invariant T (MAIT) cells, blood-Treg cells, tumor-Treg cells, and exhausted CD8+ T cells, Th1-like cells, Th17 cells, follicular T helper and T regulatory cells and tissue-resident memory T cells. The gene discussed is CD4; the disease is colorectal carcinoma.