Membrane-associated overexpression of TMEM88 in A549, H1299, H460, H292, SPC-A1, LTEP-A-2, LK2, PG-BE1, and PG-LH7 cell lines was found to inhibit the Wnt/β-catenin pathway, by reducing the expression of its target genes cyclin D1, c-Myc, and MMP7, consistently, a decreased proliferation, colony formation, migration, and invasion, as well as a decrease in tumor growth in vivo was observed. The gene discussed is CCND1; the disease is neoplasm.