To characterize the effects of FLT3-ITD on glucose metabolism, we first analyzed a panel of eight different AML cell lines (THP1, HEL, HL60, U937, MV4-11, Molm13, Molm14, PL21) by nuclear magnetic resonance (NMR) spectroscopy to assess their mitochondrial TCA cycle and their glycolytic activity, taking the ratio of the concentrations of the tricarboxylic acid (TCA) cycle intermediates fumarate to lactate (Fig. 1A) and succinate to lactate as indicators (Fig. 1B). Here, FLT3 is linked to acute myeloid leukemia.