Across the entire breast cancer cohort, the median TMBs were 27.0 mut in ER+/HER2−, 37.5 mut in HER2+/ER+, 40.0 mut in HER2+/ER− and 49.5 mut in TNBC (Supplementary Fig. S4B). The gene discussed is ESR1; the disease is breast carcinoma.