On the contrary, the relative abundance of PCSK9 and NLRP3 positively correlated with obesity diagnosis indices (body weight, fat mass and Lee’s index), abnormal sperm rate, pyroptosis markers including Caspase-1, GSDMD, IL-1, IL-18 and the level of lipid metabolizing hormone (TG, TC and LDL) (Fig. 6F). The gene discussed is GSDMD; the disease is obesity due to melanocortin 4 receptor deficiency.