To evaluate the potential role of DUSP1 in IRI-induced AKI, we generated Dusp1 knockout mice (Fig. 2A) and established AKI models with Dusp1 knockout (Dusp1−/−) and wild-type (WT) mice (bilateral ischemia for 28 min followed by reperfusion for 48 h). The gene discussed is DUSP1; the disease is acute kidney injury.