In addition, the high plasma MIF levels did not alter adipogenesis gene PPARγ and FASN expression (Figure 3K), suggesting that MIF-mediated AMPK/JNK activation in adipose tissue selectively impacted HSL and lipolysis during the development of adipocyte hypertrophy and obesity. This evidence concerns the gene MAPK8 and obesity due to melanocortin 4 receptor deficiency.