In the m5C-related module from WGCNA, we found SEPT3, CHI3L1, PLBD1, PHYHIPL, SAMD8, RAP1B, B3GNT5, RER1, PTPN7, SLC39A1 and MXI1 were prognostic factors for glioma, and they were used to construct a m5C-related signature. This evidence concerns the gene RER1 and central nervous system cancer.