Second, as a critical inhibitory transmitter, GABA exhibits anti‐inflammatory property, including the ability to diminish inflammatory responses in animal models of multiple sclerosis and cancer and LPS‐treated bovine mammary epithelial cells by inhibiting the TLR4/NF‐κB pathway.[12, 17, 19] Here we also found GABA supplementation suppressed the pro‐inflammatory response and microglia activation mediated by TLR4/NF‐κB pathway in vivo of Cntnap4 deficiency mice and in in vitro of LPS‐treated microglial BV2 cells. This evidence concerns the gene CNTNAP4 and multiple sclerosis.