To investigate the up-regulation of PRKAG2-AS in DCM samples, we analyzed the expression of heart failure markers, such as atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), β-myosin heavy chain (Myh7) and α-myosin heavy chain (Myh6), in AC16 cardiomyocytes after both PRKAG2-AS overexpression and knockdown. This evidence concerns the gene PRKAG2 and heart failure.