To investigate whether the up-regulation of PRKAG2 in dilated cardiomyopathy (DCM) is involved in heart failure, an adenovirus was constructed to overexpress PRKAG2b and transfected into primary cardiomyocytes at 10 multiplicity of infection (MOI) and 50 MOI. This evidence concerns the gene PRKAG2 and dilated cardiomyopathy.