In TCGA diffuse glioma data (n = 595, Additional file 1: Table S8), NRG3 RNA expression decreased with tumor grade in IDHmut astrocytomas and oligodendrogliomas (p < 0.05, Wilcoxon rank-sum test), especially upon progression to grade 4 astrocytomas (p < 0.0001, Wilcoxon rank-sum test) (Fig. 2h). Here, NRG3 is linked to astrocytoma (excluding glioblastoma).