Subsequently, COX survival analysis was performed, and the results of COX univariate and multivariate analysis showed that PTK6 protein expression(P = 0.039,HR = 4.507) and N stage(P = 0.011,HR = 2.432) were independent risk factors for DFS, and PTK6-positive patients were more likely to develop tumor progression, recurrence or metastasis than that PTK6-negative patients in the LNM + group, representing a poor prognosis. This evidence concerns the gene PTK6 and neoplasm.