Our study found significant associations between DNA methylation and HS6ST2 expression within 13 distinct cancer types, including negative correlations in BRCA, COAD, HNSC, KIRP, KIRC, PAAD, PRAD, LIHC, and UCEC and positive correlations in BLCA, LUSC, LUAD, and THCA. Here, HS6ST2 is linked to cancer.