Interestingly, as shown in Fig. 3A, B, quantitative analysis revealed that gene silencing of 4 of the 15 genes (ATP8B3, FOXR2, FRG2 and HIST1H4A) significantly increased tumorsphere forming efficiency compared to non-targeting sgRNA KO, highlighting these genes as potential breast cancer stemness regulators. The gene discussed is FRG2; the disease is breast carcinoma.