GSTM1 and hemoglobin H disease: Thus, single-tube multiplex real-time PCR with EvaGreen and HRM analysis may be used as an alternative for routine clinical diagnosis of α0-thal and may be especially useful for genetic counseling in prevention and control programs for severe thalassemias, including HbH disease and Hb Bart’s hydrops fetalis, in regions with a high prevalence of α-thalassemia.