SOX2 and esophageal cancer: Based on the read threshold 12 for SOX2 ChIP-seq in KYSE-70 cells set by the tangent line of slope 1 in a scaled plot of SOX2 ChIP-seq read to ranked order by increasing value, we noticed that -5SE had an enriched SOX2 binding in human esophageal cancer (Supplementary Figure 14B).