Our targeted sequencing panel, which includes the13 genes mentioned above (APC, ATM, BRCA1, BRCA2, CDH1, CHEK2, MLH1, MSH2, MSH6, PALB2, PTEN, STK11, and TP53), may be capable of evaluating germline predisposition for lung cancer, colorectal cancer, and breast cancer simultaneously (Figure S1). This evidence concerns the gene MSH2 and breast cancer.