A recent study of 40 adolescent boys aged 11–16 years with NAFLD, undergoing an 8-week feeding protocol, demonstrated that the rate of de novo lipogenesis decreased from 25% to 17% in parallel to decreases in alanine aminotransferase and hepatic steatosis, consistent with the theory that de novo lipogenesis is a critical metabolic function linking dietary sugars and NAFLD.67 This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.