Sex, age, smoking, alcohol consumption, diabetes, hypertension, cardiovascular disease, child-pugh grade, ECOG-PS score, whether B viral hepatitis, whether cirrhosis, AFP lever before treatment (< 400ng/ml or ≥400 ng/ml), HBV-DNA lever (0-1×103 or >1×103), whether giant HCC, PVTT type (I-II or III-IV), whether extrahepatic metastasis, whether PVTT was treated with radiotherapy, and whether there was reduction of PVTT were analyzed by univariate Cox analysis. The gene discussed is AFP; the disease is hepatitis B virus infection.