Therefore, following previous research, we treated HCAECs with cuproptosis inducer CuCI2 and inhibitor GSH, and observed cell viability and changes in levels of cuproptosis marker proteins HSP70, LIAS and FDX1, so as to verify whether cuproptosis affects atherosclerosis by regulating endothelial cell function. The gene discussed is FDX1; the disease is atherosclerosis.