After knockdown of CENPA, the proliferation and colony formation capabilities of the Huh7 cells were significantly inhibited (Figure 2A and 2B), whereas overexpression of CENPA in MHCC-97H and HLF cells enhanced the proliferation and colony formation capabilities of HCC cells (Figure 2A and 2B; Figure S2C and S2D). This evidence concerns the gene CENPA and hepatocellular carcinoma.