To investigate the effect of BCAR1 Tyr410 phosphorylation on FLOT1 induced gastric cancer cell proliferation, migration and invasion, we transfected WT-BCAR1 and MT-BCAR1 (Y410F) overexpression plasmids into FLOT1-KD stable AGS cell, and the abilities of cell proliferation, migration and invasion were examined. This evidence concerns the gene JAG1 and gastric cancer.