To further investigate the role of FLOT1 in gastric cancer growth and development in vivo, FLOT1-OE HGC-27 cells (FLOT1-OE group) and FLOT1-KD AGS cells (FLOT1-KD group) and corresponding control cells were inoculated into NOD-SCID mice subcutaneously. This evidence concerns the gene FLOT1 and gastric cancer.