To more accurately confirm the role of S100A16 in alcoholic fatty liver disease, S100a16f/f mice were crossed with Albumin-Cre mice to generate hepatocyte-specific S100A16 knockout mice (genotype: S100a16LKO) and control mice (genotype: S100a16f/f) (Fig. S5A-B). This evidence concerns the gene ALB and alcoholic fatty liver disease.