Consistently, qRT-PCR analysis (tumor =64, normal =64) showed that the mRNA level of TTC13 was significantly higher in ccRCC tissues than in normal tissues (P < 0.001) (Fig. 1D) which was further confirmed by western blotting (Figs. 1E and 1G) and IHC (Fig. 1F). The gene discussed is TTC13; the disease is nonpapillary renal cell carcinoma.