Interestingly, CRC cells were co-cultured with CAFs to observe whether CRC cells cause ZEB1 and MYL9 expression in CAFs, and PCR and western blotting confirmed that co-culture with CRC cells could promote the gene levels and protein expression of ZEB1 and MYL9 in CAFs (Fig. 8I, J), and the expression of ZEB1 and MYL9 in CAFs can further promote the progression of CRC. Here, ZEB1 is linked to colorectal carcinoma.