Next, to investigate whether SAA promotes stemness transformation by promoting the self-renewal of cancer stem population or by inducing dedifferentiation of other tumor cells, we sorted CD133+ cancer stem cell population and labeled them with CellTracker, subsequently, the tagged cancer stem cells were mixed again with other tumor cells and cultured in organoid medium with recombinant SAA1 or α-SAA for 5 days. This evidence concerns the gene PROM1 and cancer.