Additionally, high levels of proximity ligation assay (PLA) signals showing the interaction between hnRNPA2B1 and ALIX were observed in the cytoplasm of circTLCD4-RWDD3 overexpressing NSCLC cells compared to the control, which were reduced by hnRNPA2B1 K108 residue mutation (Fig. 5c), indicating that circTLCD4-RWDD3-induced SUMOylation of hnRNPA2B1 is required for its interaction with ALIX. This evidence concerns the gene PDCD6IP and non-small cell lung carcinoma.