We demonstrated that circTLCD4-RWDD3 bound with the nucleotides −259 to −250 of UBC9 promoter to form a DNA-RNA triplex and further activated UBC9 transcription by recruiting hnRNPA2B1 and H3K4me3 in NSCLC cells. This evidence concerns the gene HNRNPA2B1 and non-small cell lung carcinoma.