Therefore, our data elucidate that the SUMOylated hnRNPA2B1 activates ALIX to recruit ESCRT-III, thereby drive the packaging of circTLCD4-RWDD3 and hnRNPA2B1 into EVs secreted by NSCLC cells. This evidence concerns the gene HNRNPA2B1 and non-small cell lung carcinoma.