TNFRSF4 and metastatic malignant neoplasm in the lymph nodes: In addition, when positive expression in both TCs and ICs was considered to assign the expression of an immune marker as positive, OX40 positive expression was significantly associated with the presence of lymph node metastasis and advanced anatomic stage (p = 0.005 and p < 0.001, respectively), in contrast to other immune markers (Additional file 1: Table S1).