While all three variants confirmed by SP-ddPCR (i.e., LAMC3 c.1241G>A, NRXN3 c.308G>A, and ASNA1 c.193C>T) were present in the surrounding colon mucosa, they were absent in the matched cancerous tissues, advocating a negative selection during tumor evolution. The gene discussed is LAMC3; the disease is neoplasm.