It should be noted that the t-RNA and EF-Tu binding seems to be still relevant for the toxicity of SidH as mutants of SidH designed to disrupt SidH-tRNA and SidH-EF-Tu interfaces rendered SidH non-toxic in HEK cells as well as in infection (Figs. 5b,  7). This evidence concerns the gene EEF1A1 and infection.