FN1 and pancreatic ductal adenocarcinoma: We analyzed a pancreatic ductal adenocarcinoma cell line (PANC-1) and a colorectal cancer cell line (HCT116), showing that (i) cells adhered to FN, attaining the plateau value and spreading on the substrate at T90 (Fig 6A); (ii) the average Vrest of FN-seeded cells hyperpolarized, between T0 and T90, by ∼20 mV in PANC-1 and 10 mV in HCT116 (Fig 6B).