High levels of H3K9me2 repressive mark in PROM1 P1 promoter inhibit its activity and CD133 expression in glioma cells, whereas G9a (histone-lysine N-methyltransferase, H3 lysine-9 specific 3) inhibition by bix01294 increases CD133 and Sox2 expression resulting in improved sphere-forming efficiency [57]. The gene discussed is EHMT2; the disease is central nervous system cancer.