As shown in Figure 2A, unpaired sample analysis found that compared to normal samples, GPER1 mRNA expression was higher in GBM (P = 0.009), LGG (P = 0.002), HNSC, KIRC, LAML and PAAD (all P < 0.001) and lower in ACC, BLCA, BRCA, CESC, CHOL, COAD, ESCA, KICH, LUAD, LUSC, OV, PRAD, READ, PAN-CNACER, STAD, TGCT, THCA, UCEC, UCS (all P < 0.001) and PCPG (P = 0.015). The gene discussed is GPER1; the disease is glioblastoma.