Importantly, abrogating AP2 subunits α1 and μ2, as well as AAK1, significantly enhanced RAI uptake in NIS-stably expressing thyroid cancer cell lines TPC-1 (TPC-1-NIS) and 8505C (8505C-NIS) (Fig. 1C; Supp Fig. S2C), although depleting AP2σ2 had no effect. This evidence concerns the gene AAK1 and thyroid gland carcinoma.