Considering that epithelial cells, which have been identified as primary targets of viral replication in the lung and the nasal epithelium of SARS-CoV-2-infected patients (56, 60, 61), also express NLRP3 inflammasome (62), we then analyzed the activation of NLRP3 inflammasome during the infection of permissive host Caco-2 cells and ACE2-overexpressing A549 (ACE2-A549) cells. Here, ACE2 is linked to infection.