Among all adult-type diffuse gliomas combined, the optimal driver mutation VAF (either TERT for IDHwt GBM or IDH1/2 for IDHmut astrocytoma and IDHmut oligodendroglioma) in the context of MGMT promoter methylation pyrosequencing was determined by Cutoff Finder at 0.325 (Supplementary Figure S1A). Here, IDH1 is linked to astrocytoma (excluding glioblastoma).