A cohort of 6 IDHwt GBM samples with TERT VAF ≤ 0.10 (lower than VAF cutoff values identified by both Cutoff Finder and regression, Fig. 2E-H) and a sex- and age-matched control cohort with TERT VAF ≥ 0.25 (higher than both VAF cutoff values) were re-tested, using the same FFPE tissue blocks as initial pyrosequencing and NGS assays. The gene discussed is TERT; the disease is glioblastoma.