Among all adult-type diffuse gliomas combined, the optimal driver mutation VAF (either TERT for IDHwt GBM or IDH1/2 for IDHmut astrocytoma and IDHmut oligodendroglioma) in the context of MGMT promoter methylation pyrosequencing was determined by Cutoff Finder at 0.325 (Supplementary Figure S1A). The gene discussed is MGMT; the disease is astrocytoma (excluding glioblastoma).