To identify the most sensitive and specific diagnostic biomarker to be used in detecting salivary gland tumors, the study has critically introduced a detection strategy based on nanoparticles for salivary gland tumors, and evaluated diagnostic potential of CD24-AuNC (index test) compared to non-conjugated CD24 (reference test) in determination of salivary gland tumors. Here, CD24 is linked to tumor of salivary gland.