To further determine the role of JNK in NAUK1-mediated upregulation of c-Jun in ESCC cells, NUAK1-overexpressing EC109 cells were treated with two specific JNK inhibitors SP600125 and JNK-IN-8 for 24 h, and then the cell lysates were collected and subjected to Western blot analysis. This evidence concerns the gene MAPK8 and esophageal squamous cell carcinoma.