The top significant DE-genes (Padj < 0.05) between NK cells cultured alone and in co-culture with CML cells were different costimulatory genes such as TNFRSF9 (4-1BB), TNFRSF18 (GITR), TNFRSF4 (OX-40), and CRTAM, but also cytotoxicity genes like IFNG and GZMB, as also previously observed for K562 and certain other cell lines [33]. This evidence concerns the gene GZMB and chronic myelogenous leukemia, BCR-ABL1 positive.