CD8A and neoplasm: In line with the potent immunostimulatory capacity of DNA-PAE@BAY-876, cytotoxicity assay results demonstrated that pH 6.8 + DNA-PAE@BAY-876 group had the strongest anti-tumor ability, of which the 4T1 cell viability in the 4T1 + activated Treg + activated CD8+ T cell co-incubation system was only around 23% (Fig. 6h–j).