CD274 and neoplasm: Western blot analysis showed that the PD-L1 glycosylation levels decreased significantly after BAY-876 and DNA-PAE@BAY-876 treatment, substantiating the negatively regulatory role of BAY-876 on the glycosylation of tumor-intrinsic PD-L1 as well as its potential contribution to ICT enhancement (Fig. 5b, c and Supplementary Fig. 23).