From this search, as well as cross-referencing within the articles, we were able to include studies that assessed the performance of a three-gene (GBP5, DUSP3, and KLF2) signature in diagnosing tuberculosis disease compared with uninfected controls, latent tuberculosis, and other respiratory diseases, using a microbiological reference standard of a positive M tuberculosis culture, positive Xpert MTB/RIF Ultra assay, or both. This evidence concerns the gene KLF2 and tuberculosis.