According to our study findings, at the same level of gene expression analysis, patients diagnosed with HNSCC showed no significant differences in cancer grade for the genes PDGFRB, TEAD2, COL1A2, RCN1, OSBPL10, and CHCHD10. The P-value analysis, set at a significance level of P<0.05, indicated that these genes had comparable cancer grades among the HNSCC patients. The gene discussed is RCN1; the disease is head and neck squamous cell carcinoma.