ALB and variant Creutzfeldt-Jakob disease: To further increase the sensitivity of the RT-QuIC assay, a larger starting volume (200 μl) of blood was diluted in an optimised “IOME capture buffer” containing detergent, nuclease, protease inhibitors and bovine serum albumin (BSA) as a carrier protein; a modified version of the capture buffer developed for a different blood test for vCJD, the “direct detection assay” (DDA) [65].