22 over-expressed genes in cancer (2 with in vitro evidence: BIRC5, CDT1; 7 with in vivo evidence: USH1C, BLNK, RUNX2, CAV1, STIL, CDCA8, DACT1; 13 with in vivo FRAP evidence: PLK4, CBX5, G3BP1, USP42, MED1, TOPBP1, UBQLN2, CBX2, GATA3, TJP3, POU5F1, TJP1, YAP1) were identified and defined as CAL protein-coding genes since upregulated genes were more likely to be targeted (S1 Fig). The gene discussed is RUNX2; the disease is cancer.