After 21 days of CRS exposure, the mRNA expression of TLR4, MyD88, and NF-κB p65 was upregulated (TLR4, F=3.505, P=0.034; MyD88, F=3.867, P=0.025; NF-κB p65, F=4.190, P=0.019) than that of the control group (all P < 0.01), which could be downregulated after the acupuncture intervention (all P < 0.05). This evidence concerns the gene TLR4 and congenital rubella syndrome.