Finally, since GATA2 and MEIS1 have been shown to be important for HSPC function and leukemia transformation (46, 47), and their expression is upregulated in Asxl1Y588XTg cells (Figure 4E) and can be downregulated with KDM6B inhibition, we performed shRNA knockdown of GATA2 and MEIS1 in K562 and Kasumi-1 cells and examined the CFU-C frequency to establish their roles in ASXL1 mutation–mediated leukemia transformation. Here, KDM6B is linked to leukemia.