CDKL5-KO HeLa cells compared with WT cells also had decreased conversion of LC3-I to the lipidated, autophagosome-associated LC3-II after a 6-hour SINV infection, which was rescued by reconstituting CDKL5-KO cells with WT CDKL5 (Figure 1, D and E, and Supplemental Figure 1, A and B; supplemental material available online with this article; https://doi.org/10.1172/JCI168544DS1). This evidence concerns the gene CDKL5 and infection.