When we compared classified leukemia cell subsets with their normal B cell counterparts, high-dimensional phenotypes showed that gene-edited (t;411) BCP-ALL shared t-distributed stochastic neighbor embedding (t-SNE) space with corresponding patient BCP-ALL and PDX samples (Figure 1E) but not human leukemia cell lines (Supplemental Figure 2A), suggesting that the KMT2A-AFF1 gene-edited cells overall recapitulate the same phenotype and intracellular state of primary BCP-ALL cells harboring (t;411) translocation. This evidence concerns the gene AFF1 and acute lymphoblastic leukemia.