The results indicated no significant relationship between the expression level of AFAP1‐AS1 and family history, histological grade, ER, PR, HER2 status, TNBC versus TPBC in breast cancer (p > 0.05), but correlated with age (mean ± SD) (p = 0.005), TNM (p < 0.001) and lymph node metastasis (p = 0.005). The gene discussed is ESR1; the disease is breast cancer.